Functional properties of phosphorylated elongation factor 2
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چکیده
منابع مشابه
Colocalization of oxytocin and phosphorylated form of elongation factor 2 in the rat hypothalamus.
Oxytocin (OT) is one of the neuropituitary hormones and is synthesized in the neurons of the paraventricular nucleus (PVN) and supraoptic nucleus (SON). Previous studies have shown that the mRNAs encoding OT are delivered from the soma to both dendrites and axons of the neurons in the PVN and SON. However, it has not been elucidated whether a translational regulation mechanism to enable local s...
متن کاملCloning, expression and functional study of translation elongation factor 2 (EF-2) in zebrafish.
We have identified translation elongation factor 2 (EF-2) in zebrafish (GenBank Accession No. AAQ91234). Analysis of the DNA sequence of zebrafish EF-2 shows that the 2826 bp cDNA spans an open reading frame between nucleotide 55 to 2631 and encodes a protein of 858 amino acids. Zebrafish EF-2 protein shares 92%, 93%, 93% and 92% identity with the corresponding amino acid sequence in human, mou...
متن کاملEffect of propan-2-ol on enzymic and structural properties of elongation factor G.
Elongation factor G (EF-G) can support a GTPase activity in vitro even in the absence of ribosomes when propan-2-ol is present [GTPasep; De Vendittis, Masullo & Bocchini (1986) J. Biol. Chem. 261, 4445-4450]. In the present work the GTPasep activity of EF-G was further studied by investigating (i) the effect of ionic environment on GTPasep and (ii) the influence of propan-2-ol on the molecular ...
متن کاملChemical Properties of Elongation Factor 2 AMINO ACID COMPOSITIOFJ, NH,-TERMIT\rAL RESIDUE, AND SULFHYDRYL REACTIVITY
Elongation factor 2 (EF-2) and ADP ribosylated EF-2 appear to have the same amino acid composition and to consist of one polypeptide chain of approximately 1,000 residues with valine as the NH&erminal residue; the-molecular weight calculated from the composition is 110,000 per mole of either protein or adenosine-5’-diphosphate-5’-ribose (ADPR). Titration with p-hydroxymercuri[W]benzoate indicat...
متن کاملTranscription Elongation Factor GreA Has Functional Chaperone Activity
BACKGROUND Bacterial GreA is an indispensable factor in the RNA polymerase elongation complex. It plays multiple roles in transcriptional elongation, and may be implicated in resistance to various stresses. METHODOLOGY/PRINCIPAL FINDINGS In this study, we show that Escherichia coli GreA inhibits aggregation of several substrate proteins under heat shock condition. GreA can also effectively pr...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1990
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1990.tb19169.x